[HTML][HTML] Calpain expression and activity during lens fiber cell differentiation

A De Maria, Y Shi, NM Kumar, S Bassnett - Journal of biological chemistry, 2009 - ASBMB
In animal models, the dysregulated activity of calcium-activated proteases, calpains,
contributes directly to cataract formation. However, the physiological role of calpains in the
healthy lens is not well defined. In this study, we examined the expression pattern of
calpains in the mouse lens. Real time PCR and Western blotting data indicated that calpain
1, 2, 3, and 7 were expressed in lens fiber cells. Using controlled lysis, depth-dependent
expression profiles for each calpain were obtained. These indicated that, unlike calpain 1, 2 …