The FLAG™ peptide, a versatile fusion tag for the purification of recombinant proteins
A Einhauer, A Jungbauer - Journal of biochemical and biophysical …, 2001 - Elsevier
A fusion tag, called FLAG™ and consisting of eight amino acids (
AspTyrLysAspAspAspAspLys) including an enterokinase-cleavage site, was specifically
designed for immunoaffinity chromatography. It allows elution under non-denaturing
conditions [Bio/Technology, 6 (1988) 1204]. Several antibodies against this peptide have
been developed. One antibody, denoted as M1, binds the peptide in the presence of
bivalent metal cations, preferably Ca+. Elution is effected by chelating agents. Another …
AspTyrLysAspAspAspAspLys) including an enterokinase-cleavage site, was specifically
designed for immunoaffinity chromatography. It allows elution under non-denaturing
conditions [Bio/Technology, 6 (1988) 1204]. Several antibodies against this peptide have
been developed. One antibody, denoted as M1, binds the peptide in the presence of
bivalent metal cations, preferably Ca+. Elution is effected by chelating agents. Another …