[HTML][HTML] Targeting of neutral cholesterol ester hydrolase to the endoplasmic reticulum via its N-terminal sequence [S]
M Igarashi, J Osuga, M Isshiki, M Sekiya… - Journal of lipid …, 2010 - ASBMB
Neutral cholesterol ester hydrolase (NCEH) accounts for a large part of the nCEH activity in
macrophage foam cells, a hallmark of atherosclerosis, but its subcellular localization and
structure-function relationship are unknown. Here, we determined subcellular localization,
glycosylation, and nCEH activity of a series of NCEH mutants expressed in macrophages.
NCEH is a single-membrane-spanning type II membrane protein comprising three domains:
N-terminal, catalytic, and lipid-binding domains. The N-terminal domain serves as a type II …
macrophage foam cells, a hallmark of atherosclerosis, but its subcellular localization and
structure-function relationship are unknown. Here, we determined subcellular localization,
glycosylation, and nCEH activity of a series of NCEH mutants expressed in macrophages.
NCEH is a single-membrane-spanning type II membrane protein comprising three domains:
N-terminal, catalytic, and lipid-binding domains. The N-terminal domain serves as a type II …