Allergy to insect stings: II. Phospholipase A: The major allergen in honeybee venom
AK Sobotka, RM Franklin, NF Adkinson Jr… - Journal of Allergy and …, 1976 - Elsevier
In order to determine the proteins of major allergenic importance in honeybee venom (Apis
mellifera) it was chromatographed on G-50 Sephadex. The four major protein peaks eluted
were identified as hyaluronidase, phospholipase, melittin, and apamin. Testing these
preparations on the leukocytes of 6 honeybee-sensitive patients, with the in vitro method of
histamine release, revealed that all individuals were most sensitive to phospholipase A. IgE
antibodies against phospholipase A (RAST) were found in the sera of honeybee-sensitive …
mellifera) it was chromatographed on G-50 Sephadex. The four major protein peaks eluted
were identified as hyaluronidase, phospholipase, melittin, and apamin. Testing these
preparations on the leukocytes of 6 honeybee-sensitive patients, with the in vitro method of
histamine release, revealed that all individuals were most sensitive to phospholipase A. IgE
antibodies against phospholipase A (RAST) were found in the sera of honeybee-sensitive …