Continuous, clonal, insulin-and somatostatin-secreting cell lines established from a transplantable rat islet cell tumor.
AF Gazdar, WL Chick, HK Oie… - Proceedings of the …, 1980 - National Acad Sciences
AF Gazdar, WL Chick, HK Oie, HL SIMs, DL King, GC Weir, V Lauris
Proceedings of the National Academy of Sciences, 1980•National Acad SciencesContinuous cell lines that secrete both insulin and somatostatin were established by two
cooperating groups of investiagtors from a serially transplantable, radiation-induced, rat islet
cell tumor. The cell lines, named RIN-r and RIN-m, were initiated from tumors maintained in
inbred rats or in athymic nude mice, respectively. The cultured cells are epithelioid, free of
fibroblast contamination, and can be cloned. They have a hypodiploid chromosome number,
are tumorigenic, and posses amine-handling properties, including high levels of L-dopa …
cooperating groups of investiagtors from a serially transplantable, radiation-induced, rat islet
cell tumor. The cell lines, named RIN-r and RIN-m, were initiated from tumors maintained in
inbred rats or in athymic nude mice, respectively. The cultured cells are epithelioid, free of
fibroblast contamination, and can be cloned. They have a hypodiploid chromosome number,
are tumorigenic, and posses amine-handling properties, including high levels of L-dopa …
Continuous cell lines that secrete both insulin and somatostatin were established by two cooperating groups of investiagtors from a serially transplantable, radiation-induced, rat islet cell tumor. The cell lines, named RIN-r and RIN-m, were initiated from tumors maintained in inbred rats or in athymic nude mice, respectively. The cultured cells are epithelioid, free of fibroblast contamination, and can be cloned. They have a hypodiploid chromosome number, are tumorigenic, and posses amine-handling properties, including high levels of L-dopa decarboxylase and formaldehyde-induced fluorescence. Preliminary analysis of clones revealed a spectrum of insulin secretion from undetectable to relatively high. These clonal cell lines provide important systems to study the biology of insulin and somatostatin.
National Acad Sciences