[HTML][HTML] Quantitative analysis of cell composition and purity of human pancreatic islet preparations
A Pisania, GC Weir, JJ O'neil, A Omer… - Laboratory …, 2010 - Elsevier
Despite improvements in outcomes for human islet transplantation, characterization of islet
preparations remains poorly defined. This study used both light microscopy (LM) and
electron microscopy (EM) to characterize 33 islet preparations used for clinical transplants.
EM allowed an accurate identification and quantification of cell types with measured cell
number fractions (mean±sem) of 35.6±2.1% β-cells, 12.6±1.0% non-β-islet cells (48.3±2.6%
total islet cells), 22.7±1.5% duct cells, and 25.3±1.8% acinar cells. Of the islet cells …
preparations remains poorly defined. This study used both light microscopy (LM) and
electron microscopy (EM) to characterize 33 islet preparations used for clinical transplants.
EM allowed an accurate identification and quantification of cell types with measured cell
number fractions (mean±sem) of 35.6±2.1% β-cells, 12.6±1.0% non-β-islet cells (48.3±2.6%
total islet cells), 22.7±1.5% duct cells, and 25.3±1.8% acinar cells. Of the islet cells …