[HTML][HTML] Roles of thermal instability and proteolytic cleavage in regulation of activated thrombin-activable fibrinolysis inhibitor
MB Boffa, R Bell, WK Stevens, ME Nesheim - Journal of Biological …, 2000 - ASBMB
We have used site-directed mutagenesis and a recombinant expression system for thrombin-
activable fibrinolysis inhibitor (TAFI) in order to identify the thrombin cleavage site in
activated TAFI (TAFIa) and to determine the relative contribution of proteolytic cleavage and
thermal instability in regulation of TAFIa activity in clots. Arg-330 of TAFIa had been
proposed to be the thrombin cleavage site based on studies with trypsin, but mutation of this
residue to Gln did not prevent thrombin-mediated cleavage nor did mutation to Gln of the …
activable fibrinolysis inhibitor (TAFI) in order to identify the thrombin cleavage site in
activated TAFI (TAFIa) and to determine the relative contribution of proteolytic cleavage and
thermal instability in regulation of TAFIa activity in clots. Arg-330 of TAFIa had been
proposed to be the thrombin cleavage site based on studies with trypsin, but mutation of this
residue to Gln did not prevent thrombin-mediated cleavage nor did mutation to Gln of the …