[HTML][HTML] Cyclin E and SV40 small T antigen cooperate to bypass quiescence and contribute to transformation by activating CDK2 in human fibroblasts
Cyclin E overexpression is observed in multiple human tumors and linked to poor prognosis.
We have previously shown that ectopic expression of cyclin E is sufficient to induce mitogen-
independent cell cycle entry in a variety of tumor/immortal cell lines. Here we have
investigated the rate-limiting step leading to cell cycle entry in quiescent normal human
fibroblasts (NHF) ectopically expressing cyclin E. We found that in serum-starved NHF,
cyclin E forms inactive complexes with CDK2 and fails to induce DNA synthesis …
We have previously shown that ectopic expression of cyclin E is sufficient to induce mitogen-
independent cell cycle entry in a variety of tumor/immortal cell lines. Here we have
investigated the rate-limiting step leading to cell cycle entry in quiescent normal human
fibroblasts (NHF) ectopically expressing cyclin E. We found that in serum-starved NHF,
cyclin E forms inactive complexes with CDK2 and fails to induce DNA synthesis …