[HTML][HTML] Identification of eukaryotic and prokaryotic methylthiotransferase for biosynthesis of 2-methylthio-N6-threonylcarbamoyladenosine in tRNA

S Arragain, SK Handelman, F Forouhar, FY Wei… - Journal of Biological …, 2010 - ASBMB
Bacterial and eukaryotic transfer RNAs have been shown to contain hypermodified
adenosine, 2-methylthio-N 6-threonylcarbamoyladenosine, at position 37 (A 37) adjacent to
the 3′-end of the anticodon, which is essential for efficient and highly accurate protein
translation by the ribosome. Using a combination of bioinformatic sequence analysis and in
vivo assay coupled to HPLC/MS technique, we have identified, from distinct sequence
signatures, two methylthiotransferase (MTTase) subfamilies, designated as MtaB in bacterial …