[HTML][HTML] Protein kinase Cμ regulation of the JNK pathway is triggered via phosphoinositide-dependent kinase 1 and protein kinase Cε
I Brändlin, T Eiseler, R Salowsky… - Journal of Biological …, 2002 - ASBMB
The protein kinase C (PKC)-related enzyme PKCμ/PKD (protein kinase D) is activated by
activation loop phosphorylation through PKCη. Here we demonstrate that PKCμ is activated
by the direct phosphorylation of PKCε. PKCμ colocalizes with PKCε in HEK293 and MCF7
cells as shown by confocal immunofluorescence analyses. PDK1, known as the upstream
kinase for several PKC isozymes, associates intracellularly with PKCε and PKCη. PKCη is
phosphorylated by PDK1 in vitro, leading to kinase activation as similarly reported for PKCε …
activation loop phosphorylation through PKCη. Here we demonstrate that PKCμ is activated
by the direct phosphorylation of PKCε. PKCμ colocalizes with PKCε in HEK293 and MCF7
cells as shown by confocal immunofluorescence analyses. PDK1, known as the upstream
kinase for several PKC isozymes, associates intracellularly with PKCε and PKCη. PKCη is
phosphorylated by PDK1 in vitro, leading to kinase activation as similarly reported for PKCε …