[HTML][HTML] Regulation of Foxo-1 and the angiopoietin-2/Tie2 system by shear stress

S Chlench, NM Disassa, M Hohberg, C Hoffmann… - FEBS letters, 2007 - Elsevier
S Chlench, NM Disassa, M Hohberg, C Hoffmann, T Pohlkamp, G Beyer, M Bongrazio…
FEBS letters, 2007Elsevier
Transcription factor Foxo-1 can be inactivated via Akt-mediated phosphorylation. Since
shear stress activates Akt, we determined whether Foxo-1 and the Foxo-1-dependent,
angiogenesis-related Ang-2/Tie2-system are influenced by shear stress in endothelial cells.
Expression of Foxo-1 and its target genes p27Kip1 and Ang-2 was decreased under shear
stress (6dyn/cm2, 24h), nuclear exclusion of Foxo-1 by phosphorylation increased. eNOS
and Tie2 were upregulated. No effects on Ang-1 expression were detected. In conclusion …
Transcription factor Foxo-1 can be inactivated via Akt-mediated phosphorylation. Since shear stress activates Akt, we determined whether Foxo-1 and the Foxo-1-dependent, angiogenesis-related Ang-2/Tie2-system are influenced by shear stress in endothelial cells. Expression of Foxo-1 and its target genes p27Kip1 and Ang-2 was decreased under shear stress (6dyn/cm2, 24h), nuclear exclusion of Foxo-1 by phosphorylation increased. eNOS and Tie2 were upregulated. No effects on Ang-1 expression were detected. In conclusion, Foxo-1 and Ang-2/Tie2 are part of the molecular response to shear stress, which may regulate angiogenesis.
Elsevier