[HTML][HTML] Phosphorylation of collapsin response mediator protein-2 by Rho-kinase: evidence for two separate signaling pathways for growth cone collapse
We previously identified Rho-associated protein kinase (Rho-kinase) as a specific effector of
Rho. In this study, we identified collapsin response mediator protein-2 (CRMP-2), as a novel
Rho-kinase substrate in the brain. CRMP-2 is a neuronal protein whose expression is up-
regulated during development. Rho-kinase phosphorylated CRMP-2 at Thr-555 in vitro. We
produced an antibody that specifically recognizes CRMP-2 phosphorylated at Thr-555.
Using this antibody, we found that Rho-kinase phosphorylated CRMP-2 downstream of Rho …
Rho. In this study, we identified collapsin response mediator protein-2 (CRMP-2), as a novel
Rho-kinase substrate in the brain. CRMP-2 is a neuronal protein whose expression is up-
regulated during development. Rho-kinase phosphorylated CRMP-2 at Thr-555 in vitro. We
produced an antibody that specifically recognizes CRMP-2 phosphorylated at Thr-555.
Using this antibody, we found that Rho-kinase phosphorylated CRMP-2 downstream of Rho …