Intestinal epithelial cell surface membrane glycoprotein synthesis: II. Glycosyltransferases and endogenous acceptors of the undifferentiated cell surface membrane

MM Weiser - Journal of Biological Chemistry, 1973 - ASBMB
The cell surface membranes of mitotically active crypt cells from rat intestinal epithelium
were found to have glycosyltransferase activities and endogenous acceptors not found on
the differentiating villus cells. Rat intestinal epithelial cells were isolated as intact cells from
different levels of the villus and crypt areas representing sequential stages of cellular
differentiation. Incubation of these cells with UDP-N-acetyl [1-14 C] glucosamine, UDP-[U-14
C] glucose, UDP-[1-3 H] galactose, GDP-[U-14 C] mannose, and GDP-[U-14 C] fucose …