Printed covalent glycan array for ligand profiling of diverse glycan binding proteins
O Blixt, S Head, T Mondala… - Proceedings of the …, 2004 - National Acad Sciences
O Blixt, S Head, T Mondala, C Scanlan, ME Huflejt, R Alvarez, MC Bryan, F Fazio…
Proceedings of the National Academy of Sciences, 2004•National Acad SciencesHere we describe a glycan microarray constructed by using standard robotic microarray
printing technology to couple amine functionalized glycans to an amino-reactive glass slide.
The array comprises 200 synthetic and natural glycan sequences representing major glycan
structures of glycoproteins and glycolipids. The array has remarkable utility for profiling the
specificity of a diverse range of glycan binding proteins, including C-type lectins, siglecs,
galectins, anticarbohydrate antibodies, lectins from plants and microbes, and intact viruses.
printing technology to couple amine functionalized glycans to an amino-reactive glass slide.
The array comprises 200 synthetic and natural glycan sequences representing major glycan
structures of glycoproteins and glycolipids. The array has remarkable utility for profiling the
specificity of a diverse range of glycan binding proteins, including C-type lectins, siglecs,
galectins, anticarbohydrate antibodies, lectins from plants and microbes, and intact viruses.
Here we describe a glycan microarray constructed by using standard robotic microarray printing technology to couple amine functionalized glycans to an amino-reactive glass slide. The array comprises 200 synthetic and natural glycan sequences representing major glycan structures of glycoproteins and glycolipids. The array has remarkable utility for profiling the specificity of a diverse range of glycan binding proteins, including C-type lectins, siglecs, galectins, anticarbohydrate antibodies, lectins from plants and microbes, and intact viruses.
National Acad Sciences