siRNA-directed inhibition of HIV-1 infection

CD Novina, MF Murray, DM Dykxhoorn, PJ Beresford… - Nature medicine, 2002 - nature.com
CD Novina, MF Murray, DM Dykxhoorn, PJ Beresford, J Riess, SK Lee, RG Collman
Nature medicine, 2002nature.com
RNA interference silences gene expression through short interfering 21–23-mer double-
strand RNA segments that guide mRNA degradation in a sequence-specific fashion. Here
we report that siRNAs inhibit virus production by targeting the mRNAs for either the HIV-1
cellular receptor CD4, the viral structural Gag protein or green fluorescence protein
substituted for the Nef regulatory protein. siRNAs effectively inhibit pre-and/or post-
integration infection events in the HIV-1 life cycle. Thus, siRNAs may have potential for …
Abstract
RNA interference silences gene expression through short interfering 21–23-mer double-strand RNA segments that guide mRNA degradation in a sequence-specific fashion. Here we report that siRNAs inhibit virus production by targeting the mRNAs for either the HIV-1 cellular receptor CD4, the viral structural Gag protein or green fluorescence protein substituted for the Nef regulatory protein. siRNAs effectively inhibit pre- and/or post-integration infection events in the HIV-1 life cycle. Thus, siRNAs may have potential for therapeutic intervention in HIV-1 and other viral infections.
nature.com