Analysis of creatinine in mouse and rat serum by ion exchange high performance liquid chromatography for in vivo studies of renal function
KJ Fountain, A Kloss, I Garibyan, B Blitshteyn… - … of Chromatography B, 2007 - Elsevier
An ion exchange high performance liquid chromatography method was developed for
determining creatinine levels in both mouse and rat serum samples. Separation of creatinine
from other serum components was achieved in 10min using a 100× 4.1-mm, 10μm strong
cation exchange column following acetonitrile precipitation of serum proteins. Incorporation
of a guard cartridge placed in-line prior to the analytical column was employed to prevent
interference from compounds used in renal disease animal trials. Creatinine levels in normal …
determining creatinine levels in both mouse and rat serum samples. Separation of creatinine
from other serum components was achieved in 10min using a 100× 4.1-mm, 10μm strong
cation exchange column following acetonitrile precipitation of serum proteins. Incorporation
of a guard cartridge placed in-line prior to the analytical column was employed to prevent
interference from compounds used in renal disease animal trials. Creatinine levels in normal …