Identification and expression cloning of a leptin receptor, OB-R

LA Tartaglia, M Dembski, X Weng, N Deng… - Cell, 1995 - cell.com
LA Tartaglia, M Dembski, X Weng, N Deng, J Culpepper, R Devos, GJ Richards…
Cell, 1995cell.com
The ob gene product, leptin, is an important circulating signal for the regulation of body
weight. To identify high affinity leptin-binding sites, we generated a series of leptin-alkaline
phosphatase (AP) fusion proteins as well as [i251] leptin. After a binding survey of cell lines
and tissues, we identified leptin-binding sites in the mouse choroid plexus. A cDNA
expression library was prepared from mouse choroid plexus and screened with a leptin-AP
fusion protein to identify a leptin receptor (OB-R). OB-R is a single membrane-spanning …
Summary
The ob gene product, leptin, is an important circulating signal for the regulation of body weight. To identify high affinity leptin-binding sites, we generated a series of leptin-alkaline phosphatase (AP) fusion proteins as well as [i251] leptin. After a binding survey of cell lines and tissues, we identified leptin-binding sites in the mouse choroid plexus. A cDNA expression library was prepared from mouse choroid plexus and screened with a leptin-AP fusion protein to identify a leptin receptor (OB-R). OB-R is a single membrane-spanning receptor most related to the gp130 signal-transducing component of the IL-6 receptor, the G-CSF receptor, and the LIF receptor. OB-R mRNA is expressed not only in choroid plexus, but also in several other tissues, including hypothalamus. Genetic mapping of the gene encoding OB-R shows that it is within the 5.1 CM interval of mouse chromosome 4 that contains the db locus.
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