[HTML][HTML] p42/p44 mitogen-activated protein kinases phosphorylate hypoxia-inducible factor 1α (HIF-1α) and enhance the transcriptional activity of HIF-1
DE Richard, E Berra, E Gothié, D Roux… - Journal of Biological …, 1999 - ASBMB
Hypoxia-inducible factor-1 (HIF-1) controls the expression of a number of genes such as
vascular endothelial growth factor and erythropoietin in low oxygen conditions. However, the
molecular mechanisms that underlie the activation of the limiting subunit, HIF-1α, are still
poorly resolved. Results showing that endogenous HIF-1α migrated 12 kDa higher than in
vitrotranslated protein led us to evaluate the possible role of phosphorylation on this
phenomenon. We report here that HIF-1α is strongly phosphorylated in vivo and that …
vascular endothelial growth factor and erythropoietin in low oxygen conditions. However, the
molecular mechanisms that underlie the activation of the limiting subunit, HIF-1α, are still
poorly resolved. Results showing that endogenous HIF-1α migrated 12 kDa higher than in
vitrotranslated protein led us to evaluate the possible role of phosphorylation on this
phenomenon. We report here that HIF-1α is strongly phosphorylated in vivo and that …