[HTML][HTML] Gating connexin 43 channels reconstituted in lipid vesicles by mitogen-activated protein kinase phosphorylation
DY Kim, Y Kam, SK Koo, CO Joe - Journal of Biological Chemistry, 1999 - ASBMB
The regulation of gap junctional permeability by phosphorylation was examined in a model
system in which connexin 43 (Cx43) gap junction hemichannels were reconstituted in lipid
vesicles. Cx43 was immunoaffinity-purified from rat brain, and Cx43 channels were
reconstituted into unilamellar phospholipid liposomes. The activities of the reconstituted
channels were measured by monitoring liposome permeability. Liposomes containing the
Cx43 protein were fractionated on the basis of permeability to sucrose using sedimentation …
system in which connexin 43 (Cx43) gap junction hemichannels were reconstituted in lipid
vesicles. Cx43 was immunoaffinity-purified from rat brain, and Cx43 channels were
reconstituted into unilamellar phospholipid liposomes. The activities of the reconstituted
channels were measured by monitoring liposome permeability. Liposomes containing the
Cx43 protein were fractionated on the basis of permeability to sucrose using sedimentation …