The expression of a metallothionein-ovine growth hormone fusion gene in transgenic mice does not impair fertility but results in pathological lesions in the liver

JM ORIAN, C SEONG LEE, LM WEISS… - …, 1989 - academic.oup.com
JM ORIAN, C SEONG LEE, LM WEISS, MR BRANDON
Endocrinology, 1989academic.oup.com
The physiological effects of high serum levels of ovine GH (oGH) were studied in three
generations of transgenic mice carrying a metallothionein l-(MT) oGH fusion gene. Livers of
mice expressing oGH were enlarged, irrespective of the level of serum oGH detected. In
mice expressing high levels of oGH, direct measurements of hepatocytes in liver sections
revealed that cell and nuclear size were abnormally large. Hepatocytes of different
transgenic mice varied from 1.4–2.2 times normal size and hepatocyte nuclei varied from 1.7 …
The physiological effects of high serum levels of ovine GH (oGH) were studied in three generations of transgenic mice carrying a metallothionein l-(MT)oGH fusion gene. Livers of mice expressing oGH were enlarged, irrespective of the level of serum oGH detected. In mice expressing high levels of oGH, direct measurements of hepatocytes in liver sections revealed that cell and nuclear size were abnormally large. Hepatocytes of different transgenic mice varied from 1.4–2.2 times normal size and hepatocyte nuclei varied from 1.7–2.4 times normal size. In addition, intranuclear inclusions were observed in hepatocytes of transgenic mice and their presence was always associated with high serum levels of oGH. In contrast to female transgenic mice containing mouse MT-human, rat, or bovine GH fusion genes female mice containing the MT oGH fusion gene were fertile and their pituitary glands showed synthesis of GH. (Endocrinology124: 455–463, 1989)
Oxford University Press