c-Jun enhancement of androgen receptor transactivation is associated with prostate cancer cell proliferation

SY Chen, C Cai, CJ Fisher, Z Zheng, J Omwancha… - Oncogene, 2006 - nature.com
SY Chen, C Cai, CJ Fisher, Z Zheng, J Omwancha, CL Hsieh, L Shemshedini
Oncogene, 2006nature.com
Androgens and the androgen receptor (AR) are involved in the growth and progression of
prostate cancer. Our previous studies suggest that the proto-oncoprotein c-Jun is an AR
coactivator that stimulates AR transactivation by mediating receptor dimerization and
subsequent DNA binding. To study the physiological relevance of this c-Jun activity on AR,
we have generated stable LNCaP cell lines expressing different levels of c-Jun. These cell
lines exhibit a direct correlation between endogenous c-Jun levels and AR transcriptional …
Abstract
Androgens and the androgen receptor (AR) are involved in the growth and progression of prostate cancer. Our previous studies suggest that the proto-oncoprotein c-Jun is an AR coactivator that stimulates AR transactivation by mediating receptor dimerization and subsequent DNA binding. To study the physiological relevance of this c-Jun activity on AR, we have generated stable LNCaP cell lines expressing different levels of c-Jun. These cell lines exhibit a direct correlation between endogenous c-Jun levels and AR transcriptional activity and expression of endogenous androgen-regulated genes. Disruption by antisense RNA of endogenous c-Jun expression in LNCaP cells strongly compromises the androgen-dependent proliferation of these cells. In contrast, expression of a c-Jun mutant, which is fully active in coactivation of AR but deficient in AP-1 transactivation, significantly enhances androgen-dependent proliferation. This finding indicates that the coactivation function of c-Jun is sufficient for regulating androgen-induced growth of LNCaP cells. c-Jun also enhances AR transactivtion in androgen-independent LNCaP cells, which closely mimic hormone-refractory prostate cancer cells in gene expression and growth behavior. Importantly, siRNA-mediated repression of endogenous c-Jun expression results in markedly reduced growth of these cells, strongly suggesting an important biological role for c-Jun in hormone-refractory prostate cancer.
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