Alternative splicing of RNAs transcribed from the human abl gene and from the bcr-abl fused gene

E Shtivelman, B Lifshitz, RP Gale, BA Roe, E Canaani - Cell, 1986 - cell.com
E Shtivelman, B Lifshitz, RP Gale, BA Roe, E Canaani
Cell, 1986cell.com
The primary structure of normal abl protein was determined by sequencing the coding
region of its cDNA. abl contains two alternative 5'exons spliced to a common set of 3'exons
to yield the two major abl RNA transcripts. These transcripts initiate in different promoter
regions and give rise to proteins that vary in their N-termini. In the human cell line K562, abl
is translocated from chromosome 9 to within the bcr gene on chromosome 22. Within the
fused bcr-abl gene, abl exon II alternatively splices to two adjacent bcr exons. This …
Summary
The primary structure of normal abl protein was determined by sequencing the coding region of its cDNA. abl contains two alternative 5’exons spliced to a common set of 3’exons to yield the two major abl RNA transcripts. These transcripts initiate in different promoter regions and give rise to proteins that vary in their N-termini. In the human cell line K562, abl is translocated from chromosome 9 to within the bcr gene on chromosome 22. Within the fused bcr-abl gene, abl exon II alternatively splices to two adjacent bcr exons. This phenomenon is seen in many patients with chronic myeloid leukemia.
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