Synergistic effect of tumor necrosis factor-α and interferon-γ on collagen synthesis of human skin fibroblasts in vitro

K Scharffetter, M Heckmann, A Hatamochi… - Experimental cell …, 1989 - Elsevier
K Scharffetter, M Heckmann, A Hatamochi, C Mauch, B Stein, G Riethmüller…
Experimental cell research, 1989Elsevier
The effect of tumor necrosis factor-α (TNFα) and interferon-γ (IFNγ) on collagen metabolism
by human diploid fibroblasts in confluent monolayer culture was examined. Recombinant
TNFα reduced collagen mRNA levels 2-fold and stimulated collagenase mRNA levels 5-fold,
while recombinant IFNγ affected only collagen mRNA levels. The combination of TNFα (10
ng/ml) and IFNγ (100 ng/ml) resulted in a much stronger (about 30-fold) reduction of
collagen mRNA levels indicating that the two cytokines act synergistically. In contrast no …
Abstract
The effect of tumor necrosis factor-α (TNFα) and interferon-γ (IFNγ) on collagen metabolism by human diploid fibroblasts in confluent monolayer culture was examined. Recombinant TNFα reduced collagen mRNA levels 2-fold and stimulated collagenase mRNA levels 5-fold, while recombinant IFNγ affected only collagen mRNA levels. The combination of TNFα (10 ng/ml) and IFNγ (100 ng/ml) resulted in a much stronger (about 30-fold) reduction of collagen mRNA levels indicating that the two cytokines act synergistically. In contrast no such synergism was observed with respect to collagenase mRNA levels. The effect of TNFα and IFNγ on collagen metabolism reported here indicates a complex interaction of different cytokines in the control of tissue remodeling that occurs during inflammation, repair, or atrophy.
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