MAK‐V/Hunk is a MARK/Par‐1‐related protein kinase, whose function is unknown. We studied the subcellular localization of MAK‐V/Hunk in COS‐1 cells by immunofluorescence. It has a nucleocytoplasmic distribution and is localized to the centrosome, as indicated by co‐localization with γ‐tubulin. A putative kinase‐deficient mutant, with a mutation in the invariant lysine residue in the catalytic domain, was not targeted to the nucleus or centrosome. These results suggest that the nuclear and centrosomal targeting of MAK‐V/Hunk is specific, and is likely to be coupled to its catalytic activity.