[HTML][HTML] Niacin noncompetitively inhibits DGAT2 but not DGAT1 activity in HepG2 cells1
SH Ganji, S Tavintharan, D Zhu, Y Xing… - Journal of lipid …, 2004 - ASBMB
Niacin is a widely used lipid-regulating agent in dyslipidemic patients. Previously, we have
shown that niacin inhibits triacylglycerol synthesis. In this report, using HepG2 cells, we have
examined the effect of niacin on the mRNA expression and microsomal activity of
diacylglycerol acyltransferase 1 and 2 (DGAT1 and DGAT2), the last committed but distinctly
different enzymes for triglyceride synthesis. Addition of niacin to the DGAT assay reaction
mixture dose-dependently (0–3 mM) inhibited DGAT activity by 35–50%, and the IC 50 was …
shown that niacin inhibits triacylglycerol synthesis. In this report, using HepG2 cells, we have
examined the effect of niacin on the mRNA expression and microsomal activity of
diacylglycerol acyltransferase 1 and 2 (DGAT1 and DGAT2), the last committed but distinctly
different enzymes for triglyceride synthesis. Addition of niacin to the DGAT assay reaction
mixture dose-dependently (0–3 mM) inhibited DGAT activity by 35–50%, and the IC 50 was …