Monitoring minimal residual disease with flow cytometry, antigen-receptor gene rearrangements and fusion transcript quantification in Philadelphia-positive childhood …
I Thörn, J Botling, M Hermansson, G Lönnerholm… - Leukemia research, 2009 - Elsevier
In this study, we followed minimal residual disease (MRD) in eight children with Philadelphia-
positive acute lymphoblastic leukemia (Ph+ ALL) using (i) flow cytometry (FCM),(ii) real-time
quantitative PCR of IG/TCR gene rearrangements and (iii) RT-PCR detecting fusion gene
transcripts. In six of the eight cases the kinetics of MRD clearance was comparable. One of
the two discordant cases could be explained by presence of an alternative fusion transcript.
The other discordant case showed high BCR-ABL1 RNA level while the other methods did …
positive acute lymphoblastic leukemia (Ph+ ALL) using (i) flow cytometry (FCM),(ii) real-time
quantitative PCR of IG/TCR gene rearrangements and (iii) RT-PCR detecting fusion gene
transcripts. In six of the eight cases the kinetics of MRD clearance was comparable. One of
the two discordant cases could be explained by presence of an alternative fusion transcript.
The other discordant case showed high BCR-ABL1 RNA level while the other methods did …