Steps toward mapping the human vasculature by phage display

W Arap, MG Kolonin, M Trepel, J Lahdenranta… - Nature medicine, 2002 - nature.com
W Arap, MG Kolonin, M Trepel, J Lahdenranta, M Cardó-Vila, RJ Giordano, PJ Mintz…
Nature medicine, 2002nature.com
The molecular diversity of receptors in human blood vessels remains largely unexplored.
We developed a selection method in which peptides that home to specific vascular beds are
identified after administration of a peptide library. Here we report the first in vivo screening of
a peptide library in a patient. We surveyed 47,160 motifs that localized to different organs.
This large-scale screening indicates that the tissue distribution of circulating peptides is
nonrandom. High-throughput analysis of the motifs revealed similarities to ligands for …
Abstract
The molecular diversity of receptors in human blood vessels remains largely unexplored. We developed a selection method in which peptides that home to specific vascular beds are identified after administration of a peptide library. Here we report the first in vivo screening of a peptide library in a patient. We surveyed 47,160 motifs that localized to different organs. This large-scale screening indicates that the tissue distribution of circulating peptides is nonrandom. High-throughput analysis of the motifs revealed similarities to ligands for differentially expressed cell-surface proteins, and a candidate ligand–receptor pair was validated. These data represent a step toward the construction of a molecular map of human vasculature and may have broad implications for the development of targeted therapies.
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