[HTML][HTML] Identification of parameters required for efficient lentiviral vector transduction and engraftment of human cord blood CD34+ NOD/SCID-repopulating cells
Y Liu, G Hangoc, TB Campbell, M Goodman… - Experimental …, 2008 - Elsevier
OBJECTIVE: Human cord blood (CB) is a potential source of hematopoietic stem cells (HSC)
for gene therapy to treat patients with hematopoietic disorders. However, limited numbers of
CB CD34+ cells, low transduction efficiency with lentiviral vectors (LVs), and low
engraftment efficiency of nonobese diabetic/severe combined immunodeficient (NOD/SCID)
repopulating cells (SRC), a measure of HSC, are blocks to this procedure. To optimize
culture and transduction conditions, we compared various lengths of time for prestimulation …
for gene therapy to treat patients with hematopoietic disorders. However, limited numbers of
CB CD34+ cells, low transduction efficiency with lentiviral vectors (LVs), and low
engraftment efficiency of nonobese diabetic/severe combined immunodeficient (NOD/SCID)
repopulating cells (SRC), a measure of HSC, are blocks to this procedure. To optimize
culture and transduction conditions, we compared various lengths of time for prestimulation …