[PDF][PDF] Glycogen synthase: a new activity ratio assay expressing a high sensitivity to the phosphorylation state
JJ Guinovart, A Salavert, J Massagué, CJ Ciudad… - FEBS letters, 1979 - core.ac.uk
JJ Guinovart, A Salavert, J Massagué, CJ Ciudad, E Salsas, E Itarte
FEBS letters, 1979•core.ac.ukSince its introduction [I] the-glucose 6-phosphate/t glucose 6-phosphate (-G6P/tG6P) activity
ratio assay for glycogen synthase has been a very useful tool in the study of the activation
state of this enzyme. The idea of glycogen synthase in two forms (one active in the absence
of added G6P (I form), the other requiring it for activity (D form)) evolved from the first
experiments in which the-G6P/tG6P assay was employed [11. Shortly afterwards changes in
the-G6P/tG6P activity ratio were correlated with phosphorylation and dephosphorylation of …
ratio assay for glycogen synthase has been a very useful tool in the study of the activation
state of this enzyme. The idea of glycogen synthase in two forms (one active in the absence
of added G6P (I form), the other requiring it for activity (D form)) evolved from the first
experiments in which the-G6P/tG6P assay was employed [11. Shortly afterwards changes in
the-G6P/tG6P activity ratio were correlated with phosphorylation and dephosphorylation of …
Since its introduction [I] the-glucose
6-phosphate/t glucose 6-phosphate (-G6P/tG6P) activity ratio assay for glycogen synthase has been a very useful tool in the study of the activation state of this enzyme. The idea of glycogen synthase in two forms (one active in the absence of added G6P (I form), the other requiring it for activity (D form)) evolved from the first experiments in which the
-G6P/tG6P assay was employed [11. Shortly afterwards changes in the-G6P/tG6P activity ratio were correlated with phosphorylation and dephosphorylation of the enzyme [2]. At concentrations of metabolites close to their physiological levels it was shown [3] that the D form would be inactive whereas the I form would be active.
core.ac.uk