[HTML][HTML] Unraveling Δ1-pyrroline-5-carboxylate-proline cycle in plants by uncoupled expression of proline oxidation enzymes
The two-step oxidation of proline in all eukaryotes is performed at the inner mitochondrial
membrane by the consecutive action of proline dehydrogenase (ProDH) that produces Δ 1-
pyrroline-5-carboxylate (P5C) and P5C dehydrogenase (P5CDH) that oxidizes P5C to
glutamate. This catabolic route is down-regulated in plants during osmotic stress, allowing
free Pro accumulation. We show here that overexpression of MsProDH in tobacco and
Arabidopsis or impairment of P5C oxidation in the Arabidopsis p5cdh mutant did not change …
membrane by the consecutive action of proline dehydrogenase (ProDH) that produces Δ 1-
pyrroline-5-carboxylate (P5C) and P5C dehydrogenase (P5CDH) that oxidizes P5C to
glutamate. This catabolic route is down-regulated in plants during osmotic stress, allowing
free Pro accumulation. We show here that overexpression of MsProDH in tobacco and
Arabidopsis or impairment of P5C oxidation in the Arabidopsis p5cdh mutant did not change …