Preparation and assay of mammalian thioredoxin and thioredoxin reductase
ESJ Arnér, L Zhong, A Holmgren - Methods in enzymology, 1999 - Elsevier
Publisher Summary This chapter describes the preparation and assay of mammalian
thioredoxin and thioredoxin reductase (TrxR). The amino acid sequences of mammalian
TrxR revealed a strikingly high homology to glutathione reductase. 14, 19 The conserved
features of all the structural components of glutathione reductase are preserved in
mammalian TrxR, including a redox active disulfide motif in the N-terminal FAD region, the
NADPH binding region, and the carboxyterminal interface region that governs the …
thioredoxin and thioredoxin reductase (TrxR). The amino acid sequences of mammalian
TrxR revealed a strikingly high homology to glutathione reductase. 14, 19 The conserved
features of all the structural components of glutathione reductase are preserved in
mammalian TrxR, including a redox active disulfide motif in the N-terminal FAD region, the
NADPH binding region, and the carboxyterminal interface region that governs the …