Poly (A)-binding protein binds to A-rich sequences via RNA-binding domains 1+ 2 and 3+ 4
T Khanam, RS Muddashetty, A Kahvejian… - RNA biology, 2006 - Taylor & Francis
T Khanam, RS Muddashetty, A Kahvejian, N Sonenberg, J Brosius
RNA biology, 2006•Taylor & FrancisPoly (A) binding protein (PABP) binds non-protein-coding BC1 RNA and BC200 RNA, which
contain adenosine-rich domains. Two combinations of the four PABP RNA recognition motifs
(RRMs), RRMs 1+ 2 and RRMs 3+ 4, bind with very strong affinities to various transcripts
with long stretches of adenosine residues, whereas RRMs 2+ 3 bind weakly. While RRMs
1+ 2 preferentially bind to stretches that contain only adenosines, RRMs 3+ 4 exhibit
relatively high affinities towards sequences that are interspersed with other nucleotides …
contain adenosine-rich domains. Two combinations of the four PABP RNA recognition motifs
(RRMs), RRMs 1+ 2 and RRMs 3+ 4, bind with very strong affinities to various transcripts
with long stretches of adenosine residues, whereas RRMs 2+ 3 bind weakly. While RRMs
1+ 2 preferentially bind to stretches that contain only adenosines, RRMs 3+ 4 exhibit
relatively high affinities towards sequences that are interspersed with other nucleotides …
Poly(A) binding protein (PABP) binds non-protein-coding BC1 RNA and BC200 RNA, which contain adenosine-rich domains. Two combinations of the four PABP RNA recognition motifs (RRMs), RRMs 1+2 and RRMs 3+4, bind with very strong affinities to various transcripts with long stretches of adenosine residues, whereas RRMs 2+3 bind weakly. While RRMs 1+2 preferentially bind to stretches that contain only adenosines, RRMs 3+4 exhibit relatively high affinities towards sequences that are interspersed with other nucleotides. Binding studies with oligoribonucleotide(A)65 and oligoribonucleotide(A)25 showed that the shorter RNA is not an ideal substrate for binding studies to model the interactions with mRNAs, which in general harbor long poly(A) tails.
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