Semiquantitative immunohistochemical analysis for hypoxia in human tumors
JA Raleigh, SC Chou, EL Bono, DE Thrall… - International Journal of …, 2001 - Elsevier
Objective: The goal of this study was to develop a semiquantitative scoring system for
measuring hypoxia in human tumors by an immunohistochemical marker approach.
Methods and Materials: Eighteen patients diagnosed with squamous cell carcinoma of the
uterine cervix or head and neck were infused intravenously with a solution of pimonidazole
hydrochloride at a dose of 0.5 gm/m2. Twenty-four hours later, four biopsies on average from
each tumor were fixed in formalin, processed into paraffin blocks, and sectioned. Tissue …
measuring hypoxia in human tumors by an immunohistochemical marker approach.
Methods and Materials: Eighteen patients diagnosed with squamous cell carcinoma of the
uterine cervix or head and neck were infused intravenously with a solution of pimonidazole
hydrochloride at a dose of 0.5 gm/m2. Twenty-four hours later, four biopsies on average from
each tumor were fixed in formalin, processed into paraffin blocks, and sectioned. Tissue …