Simplified preparation of rabbit Fab fragments

A Coulter, R Harris - Journal of immunological methods, 1983 - Elsevier
Papain attached to solid-phase CH-Sepharose 4B was used to digest rabbit IgG. Protein A-
Sepharose CL-4B was used to remove undigested IgG and Fc fragments. Pure Fab
fragments free of IgG, Fc fragments and papain were readily obtained by this procedure with
a yield of about 75%. Polyacrylamide gel electrophoresis of the Fab in the presence of
sodium dodecyl sulphate gave a single band under both reducing and non-reducing
conditions. The molecular weight of the Fab determined by sedimentation equilibrium was …