Negative-stain immunoelectron-microscopic analysis of small macromolecules of immunologic significance
KH Roux - Methods, 1996 - Elsevier
Methods, 1996•Elsevier
We have demonstrated that unlabeled antibodies or their antigen-reactive fragments, Fab
and F (ab′) 2, can be readily visualized by negative-stain immunoelectron microscopy and
can be quite useful as probes for detailed epitope mapping and structural analysis of
relatively small macromolecules. In addition, information on segmental flexibility of the target
molecules can be deduced as can information on the geometry and kinetics of immune
complex formation in solution. The various target molecules used to illustrate these points …
and F (ab′) 2, can be readily visualized by negative-stain immunoelectron microscopy and
can be quite useful as probes for detailed epitope mapping and structural analysis of
relatively small macromolecules. In addition, information on segmental flexibility of the target
molecules can be deduced as can information on the geometry and kinetics of immune
complex formation in solution. The various target molecules used to illustrate these points …
We have demonstrated that unlabeled antibodies or their antigen-reactive fragments, Fab and F(ab′)2, can be readily visualized by negative-stain immunoelectron microscopy and can be quite useful as probes for detailed epitope mapping and structural analysis of relatively small macromolecules. In addition, information on segmental flexibility of the target molecules can be deduced as can information on the geometry and kinetics of immune complex formation in solution. The various target molecules used to illustrate these points include IgG, IgM, C-reactive protein, and complement receptor 1.
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