Glycolipid antigen processing for presentation by CD1d molecules

TI Prigozy, O Naidenko, P Qasba, D Elewaut, L Brossay… - Science, 2001 - science.org
TI Prigozy, O Naidenko, P Qasba, D Elewaut, L Brossay, A Khurana, T Natori, Y Koezuka…
Science, 2001science.org
The requirement for processing glycolipid antigens in T cell recognition was examined with
mouse CD1d-mediated responses to glycosphingolipids (GSLs). Although some
disaccharide GSL antigens can be recognized without processing, the responses to three
other antigens, including the disaccharide GSL Gal (α1→ 2) GalCer (Gal, galactose; GalCer,
galactosylceramide), required removal of the terminal sugars to permit interaction with the T
cell receptor. A lysosomal enzyme, α-galactosidase A, was responsible for the processing of …
The requirement for processing glycolipid antigens in T cell recognition was examined with mouse CD1d-mediated responses to glycosphingolipids (GSLs). Although some disaccharide GSL antigens can be recognized without processing, the responses to three other antigens, including the disaccharide GSL Gal(α1→2)GalCer (Gal, galactose; GalCer, galactosylceramide), required removal of the terminal sugars to permit interaction with the T cell receptor. A lysosomal enzyme, α-galactosidase A, was responsible for the processing of Gal(α1→2)GalCer to generate the antigenic monosaccharide epitope. These data demonstrate a carbohydrate antigen processing system analogous to that used for peptides and an ability of T cells to recognize processed fragments of complex glycolipids.
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