Endogenously generated hydrogen peroxide induces apoptosis via mitochondrial damage independent of NF-κB and p53 activation in bovine embryos
C Velez-Pardo, AT Morales, MJ Del Rio… - Theriogenology, 2007 - Elsevier
Hydrogen peroxide (H2O2) has been implicated as a key molecule in arresting embryonic
development; however, its mechanism of action is not fully established. The aim of the
present study was to determine the chronological generation of H2O2 from oocyte to morula,
and to examine the relationship of H2O2 with loss of mitochondrial membrane potential,
nuclear factor kappa-B (NF-κB), p53, caspase-3 activation, and cell death in bovine embryos
in vitro. Accordingly, superoxide anion radicals were detected between 32 and 120h after in …
development; however, its mechanism of action is not fully established. The aim of the
present study was to determine the chronological generation of H2O2 from oocyte to morula,
and to examine the relationship of H2O2 with loss of mitochondrial membrane potential,
nuclear factor kappa-B (NF-κB), p53, caspase-3 activation, and cell death in bovine embryos
in vitro. Accordingly, superoxide anion radicals were detected between 32 and 120h after in …