A novel method of generating prostate cancer metastases from orthotopic implants
E Corey, JE Quinn, RL Vessella - The Prostate, 2003 - Wiley Online Library
E Corey, JE Quinn, RL Vessella
The Prostate, 2003•Wiley Online LibraryBACKGROUND Spontaneous metastasis following implantation at the orthotopic site is a
highly desired feature in prostate cancer (CaP) models, since it would enable studies of
mechanisms associated with tumor cell dissemination. METHODS LuCaP 23.8 and LuCaP
35, hormone‐sensitive CaP xenografts that express PSA and the wild‐type androgen
receptor, were grown orthotopically in SCID mice. When tumor volumes reached∼ 250–500
mg, primary tumors were removed to allow micrometastases to grow. RESULTS Using this …
highly desired feature in prostate cancer (CaP) models, since it would enable studies of
mechanisms associated with tumor cell dissemination. METHODS LuCaP 23.8 and LuCaP
35, hormone‐sensitive CaP xenografts that express PSA and the wild‐type androgen
receptor, were grown orthotopically in SCID mice. When tumor volumes reached∼ 250–500
mg, primary tumors were removed to allow micrometastases to grow. RESULTS Using this …
BACKGROUND
Spontaneous metastasis following implantation at the orthotopic site is a highly desired feature in prostate cancer (CaP) models, since it would enable studies of mechanisms associated with tumor cell dissemination.
METHODS
LuCaP 23.8 and LuCaP 35, hormone‐sensitive CaP xenografts that express PSA and the wild‐type androgen receptor, were grown orthotopically in SCID mice. When tumor volumes reached ∼250–500 mg, primary tumors were removed to allow micrometastases to grow.
RESULTS
Using this procedure we generated macroscopic metastases (>20 mg) in 71% (LuCaP 23.8) and 100% (LuCaP 35) of animals, respectively.
CONCLUSIONS
These models may be used to evaluate new treatment modalities and study mechanisms associated with development of metastases. Prostate 56: 110–114, 2003. © 2003 Wiley‐Liss, Inc.
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