Quantification of replication of clinical cytomegalovirus isolates in cultured endothelial cells and fibroblasts by a focus expansion assay
C Sinzger, J Knapp, B Plachter, K Schmidt… - Journal of virological …, 1997 - Elsevier
A method for quantitative analysis of the growth properties of human cytomegalovirus
(HCMV) in various cell culture systems was developed. Recent HCMV isolates are, in most
cases cell associated, causing only limited cytopathic effect. This renders comparative
analysis of the biological properties of such isolates difficult. The focus expansion assay
described in this study is based on cocultivation of infected fibroblasts with a cell type of
choice, relying on cell mediated infectivity. The extent of replication of a given isolate in cell …
(HCMV) in various cell culture systems was developed. Recent HCMV isolates are, in most
cases cell associated, causing only limited cytopathic effect. This renders comparative
analysis of the biological properties of such isolates difficult. The focus expansion assay
described in this study is based on cocultivation of infected fibroblasts with a cell type of
choice, relying on cell mediated infectivity. The extent of replication of a given isolate in cell …