Muscle-specific calpain, p94, is degraded by autolysis immediately after translation, resulting in disappearance from muscle
H Sorimachi, N Toyama-Sorimachi, TC Saido… - Journal of Biological …, 1993 - ASBMB
We previously identified a third type of the calpain large subunit named p94 as a cDNA
whose mRNA is expressed exclusively in skeletal muscle at levels approximately 10-fold
more abundant than those of the conventional calpain subunit. Rat skeletal muscle fractions
were screened by two anti-peptide antibodies raised against two specific sequences in p94,
but the p94 protein could not be found. To examine this apparent discrepancy between the
amounts of mRNA and protein, wild-type p94 was expressed in COS cells. Although p94 …
whose mRNA is expressed exclusively in skeletal muscle at levels approximately 10-fold
more abundant than those of the conventional calpain subunit. Rat skeletal muscle fractions
were screened by two anti-peptide antibodies raised against two specific sequences in p94,
but the p94 protein could not be found. To examine this apparent discrepancy between the
amounts of mRNA and protein, wild-type p94 was expressed in COS cells. Although p94 …