Adaptation of eukaryotic cells to changing environmental conditions entails rapid regulation
of protein targeting and transport to specific organelles. Such adaptation is well exemplified
in mammalian cells exposed to nitrogen starvation that are triggered to form and transport
autophagosomes to lysosomes, thus constituting an inducible intracellular trafficking
pathway. Here we investigated the relationship between the general secretory machinery
and the autophagic pathway in Chinese hamster ovary cells grown in the absence of amino …