Human peripheral blood Vα24+ Vβ11+ NKT cells expand following administration of α‐galactosylceramide‐pulsed dendritic cells

M Okai, M Nieda, A Tazbirkova, D Horley… - Vox …, 2002 - Wiley Online Library
M Okai, M Nieda, A Tazbirkova, D Horley, A Kikuchi, S Durrant, T Takahashi, A Boyd
Vox sanguinis, 2002Wiley Online Library
Background and Objectives We have undertaken the first clinical trial involving the
administration of α‐GalactosylCeramine (α‐GalCer)‐pulsed dendritic cells (DCs) to human
subjects, to determine safety, optimal dose, optimal administration route and immunological
effects. Materials and Methods Subjects (n= 4) with metastatic malignancy received two
infusions of α‐GalCer‐pulsed DCs intravenously, and two infusions intradermally. The
percentages of Vα24 Vβ11 NKT cells in peripheral blood (PB) were determined by three …
Background and Objectives We have undertaken the first clinical trial involving the administration of α‐GalactosylCeramine (α‐GalCer)‐pulsed dendritic cells (DCs) to human subjects, to determine safety, optimal dose, optimal administration route and immunological effects.
Materials and Methods Subjects (n = 4) with metastatic malignancy received two infusions of α‐GalCer‐pulsed DCs intravenously, and two infusions intradermally. The percentages of Vα24 Vβ11 NKT cells in peripheral blood (PB) were determined by three‐colour flow cytometry and the PB NKT cell numbers were calculated using the total number of PB lymphocytes/ml determined by automated full‐blood counts.
Results No serious treatment related adverse events were observed during the study period. Administration of α‐GalCer‐pulsed DCs in vivo can significantly (P < 0·03) increase PB Vα24+ Vβ11+ NKT cell numbers above pretreatment baseline levels after the transient fall in the NKT numbers within 48 h.
Conclusions Administration of α‐GalCer‐pulsed DCs is well tolerated, modulates PB Vα24+ Vβ11+ NKT cells and may have a role in the therapy of malignancies sensitive to activities of Vα24+ Vβ11+ NKT cells, or for autoimmune diseases.
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