[HTML][HTML] Plasma membrane estrogen receptors are coupled to endothelial nitric-oxide synthase through Gαi
MH Wyckoff, KL Chambliss, C Mineo… - Journal of Biological …, 2001 - ASBMB
Estrogen causes rapid endothelial nitric oxide (NO) production because of the activation of
plasma membrane-associated estrogen receptors (ER) coupled to endothelial NO synthase
(eNOS). In the present study, we determined the role of G proteins in eNOS activation by
estrogen. Estradiol-17β (E 2, 10− 8 m) and acetylcholine (10− 5 m) caused comparable
increases in NOS activity (15 min) in intact endothelial cells that were fully blocked by
pertussis toxin (Ptox). In addition, exogenous guanosine 5′-O-(2-thiodiphosphate) inhibited …
plasma membrane-associated estrogen receptors (ER) coupled to endothelial NO synthase
(eNOS). In the present study, we determined the role of G proteins in eNOS activation by
estrogen. Estradiol-17β (E 2, 10− 8 m) and acetylcholine (10− 5 m) caused comparable
increases in NOS activity (15 min) in intact endothelial cells that were fully blocked by
pertussis toxin (Ptox). In addition, exogenous guanosine 5′-O-(2-thiodiphosphate) inhibited …