[HTML][HTML] Comparing microarray versus RT-PCR assessment of renal allograft biopsies: similar performance despite different dynamic ranges
In renal allografts, assessing gene expression can add relevant diagnostic information to
histopathology. Results can be expressed as single genes or gene sets, representing
pathogenesis-based transcript sets (PBTs): cytotoxic T-cell-associated, interferon gamma-
induced or decreased kidney parenchymal transcripts. Two technology platforms are
available: RT-PCR and microarrays. We compared RT-PCR, U133plus2. 0 microarrays and
histopathology in 86 biopsies. We compared 13 potentially diagnostic genes as RT-PCR …
histopathology. Results can be expressed as single genes or gene sets, representing
pathogenesis-based transcript sets (PBTs): cytotoxic T-cell-associated, interferon gamma-
induced or decreased kidney parenchymal transcripts. Two technology platforms are
available: RT-PCR and microarrays. We compared RT-PCR, U133plus2. 0 microarrays and
histopathology in 86 biopsies. We compared 13 potentially diagnostic genes as RT-PCR …