[HTML][HTML] Lipid raft localization regulates the cleavage specificity of protease activated receptor 1 in endothelial cells
JS Bae, L Yang, AR Rezaie - Journal of Thrombosis and Haemostasis, 2008 - Elsevier
Background: The endothelial protein C receptor (EPCR)-dependent cleavage of protease
activated receptor 1 (PAR-1) by either activated protein C (APC) or thrombin in lipid rafts
initiates protective signaling responses in endothelial cells. Objectives: To investigate the
mechanism by which APC and thrombin interact with and cleave PAR-1 in lipid rafts.
Methods: We constructed two types of PAR-1 cleavage reporter constructs in which a
secreted alkaline phosphatase (ALP) was fused to the extracellular domain of PAR-1. The …
activated receptor 1 (PAR-1) by either activated protein C (APC) or thrombin in lipid rafts
initiates protective signaling responses in endothelial cells. Objectives: To investigate the
mechanism by which APC and thrombin interact with and cleave PAR-1 in lipid rafts.
Methods: We constructed two types of PAR-1 cleavage reporter constructs in which a
secreted alkaline phosphatase (ALP) was fused to the extracellular domain of PAR-1. The …