[HTML][HTML] c-Fms tyrosine 559 is a major mediator of M-CSF-induced proliferation of primary macrophages

S Takeshita, R Faccio, J Chappel, L Zheng… - Journal of Biological …, 2007 - ASBMB
The molecular mechanisms by which binding of monocyte/macrophage colony-stimulating
factor to its receptor c-Fms promotes replication in primary macrophages are incompletely
understood, as all previous studies involved overexpression of receptor mutants in
transformed cells not endogenously expressing the receptor. To address this issue we
retrovirally expressed, in bone marrow-derived macrophages, a chimeric receptor
containing a range of tyrosine to phenylalanine mutations in the c-Fms cytoplasmic tail. We …