[HTML][HTML] Multidimensional Liquid Chromatography Separation of Intact Proteins by Chromatographic Focusing and Reversed Phase of the Human Serum Proteome …
S Sheng, D Chen, JE Van Eyk - Molecular & Cellular Proteomics, 2006 - ASBMB
In biomarker discovery, the detection of proteins with low abundance in the serum proteome
can be achieved by optimization of protein separation methods as well as selective
depletion of the higher abundance proteins such as immunoglobins (eg IgG) and albumin. A
relative newcomer to the proteomic separation arena is the commercial instrument PF2D
from Beckman Coulter that separates proteins in the first dimension using chromatofocusing
followed in line by reversed phase chromatography in the second dimension, thereby …
can be achieved by optimization of protein separation methods as well as selective
depletion of the higher abundance proteins such as immunoglobins (eg IgG) and albumin. A
relative newcomer to the proteomic separation arena is the commercial instrument PF2D
from Beckman Coulter that separates proteins in the first dimension using chromatofocusing
followed in line by reversed phase chromatography in the second dimension, thereby …