[HTML][HTML] Modulation of the activity and arachidonic acid selectivity of group X secretory phospholipase A2 by sphingolipids
DK Singh, PV Subbaiah - Journal of lipid research, 2007 - ASBMB
To investigate the role of sphingomyelin (SM) in the regulation of inflammatory reactions, we
studied its effect on the activity and fatty acid specificity of group X secretory phospholipase
A 2 (sPLA 2 X). Compared with other phospholipases, recombinant sPLA 2 X released more
arachidonate from HDL. Pretreatment of HDL with sphingomyelinase (SMase) C activated
the sPLA 2 X activity, but the release of arachidonate was stimulated less than that of
linoleate. In liposomes containing synthetic phosphatidylcholines (PCs), sPLA 2 X showed …
studied its effect on the activity and fatty acid specificity of group X secretory phospholipase
A 2 (sPLA 2 X). Compared with other phospholipases, recombinant sPLA 2 X released more
arachidonate from HDL. Pretreatment of HDL with sphingomyelinase (SMase) C activated
the sPLA 2 X activity, but the release of arachidonate was stimulated less than that of
linoleate. In liposomes containing synthetic phosphatidylcholines (PCs), sPLA 2 X showed …