Functionalβ-adrenergic receptors in breast cancer cells
B Vandewalle, F Revillion, J Lefebvre - Journal of cancer research and …, 1990 - Springer
B Vandewalle, F Revillion, J Lefebvre
Journal of cancer research and clinical oncology, 1990•SpringerSummary Using l-[3 H] dihydroalprenolol ([3 H] DHA), a potent β-adrenergic antagonist, we
demonstrated in breast cancer cells the presence of β-adrenergic receptors with high affinity
(K d 1–9 n M) as shown by Scatchard analyses. Natural and synthetic agonists inhibited the
[3 H] DHA binding in the following order of potency: l-isoproterenol= l epinephrine>>> l-
norepinephrine, identical to the well-established order of potency for these compounds in
producing β-adrenergic responses. We verified that these compounds actually stimulated …
demonstrated in breast cancer cells the presence of β-adrenergic receptors with high affinity
(K d 1–9 n M) as shown by Scatchard analyses. Natural and synthetic agonists inhibited the
[3 H] DHA binding in the following order of potency: l-isoproterenol= l epinephrine>>> l-
norepinephrine, identical to the well-established order of potency for these compounds in
producing β-adrenergic responses. We verified that these compounds actually stimulated …
Summary
Usingl-[3H]dihydroalprenolol ([3H]DHA), a potentβ-adrenergic antagonist, we demonstrated in breast cancer cells the presence ofβ-adrenergic receptors with high affinity (K d 1–9 nM) as shown by Scatchard analyses. Natural and synthetic agonists inhibited the [3H] DHA binding in the following order of potency:l-isoproterenol=l epinephrine > > >l-norepinephrine, identical to the well-established order of potency for these compounds in producingβ-adrenergic responses. We verified that these compounds actually stimulated cAMP production in breast cancer cells. At the present time, the pathophysiological significance ofβ-adrenergic receptors remains unclear. In view of the importance of cAMP in lactose production and in tumor growth mechanisms, it seems to be important to characterize theβ-adrenergic receptors in breast cancer cells in more detail and study their possible involvement in breast tumor growth.
Springer