Bacterial invasion and transcytosis in transfected human brain microvascular endothelial cells
MF Stins, J Badger, KS Kim - Microbial pathogenesis, 2001 - Elsevier
Most cases of neonatal bacterial meningitis develop as a result of a hematogenous spread,
but it is not clear how circulating bacteria cross the blood–brain barrier. Attempts to answer
these questions have been hampered by the lack of a reliable model of the human blood–
brain barrier. Human brain microvascular endothelial cells (HBMEC) were isolated and
transfected with a pBR322 based plasmid containing simian virus 40 large T antigen (SV40-
LT). The transfected HBMEC exhibited similar brain endothelial cell characteristics as the …
but it is not clear how circulating bacteria cross the blood–brain barrier. Attempts to answer
these questions have been hampered by the lack of a reliable model of the human blood–
brain barrier. Human brain microvascular endothelial cells (HBMEC) were isolated and
transfected with a pBR322 based plasmid containing simian virus 40 large T antigen (SV40-
LT). The transfected HBMEC exhibited similar brain endothelial cell characteristics as the …